03-DYNA-F-03: Cell biology of messenger RNA biogenesis
Abstract:
Major events in the life cycle of a messenger RNA (Mrna) include transcription, splicing, 3’ end processing, export from the nucleus to the cytoplasm, translation and degradation. These processes are intimately linked through proteins that bind to the mRNA in a specific and coordinated fashion. During the lifetime of an mRNA, the composition of associated protein complexes is under constant change. Through this Network the participating teams wish to study the dynamics of mRNA biogenesis making use of a wide range of multidisciplinary approaches. These include yeast genetics, molecular biology, structural biology, biochemical assays, proteomics, DNA microarrays, RNA interference, and live cell microscopy. The first Work Package of this proposal aims to dissect functional interactions between transcription initiation, RNA polymerase II, quality control and pre-mRNA processing events. The second Work Package is focused on the dynamics of pre-mRNA processing machines. The third Work Package aims to investigate the functional relevance of shuttling between the nucleus and the cytoplasm of proteins involved in mRNA biogenesis.
Project Leader:
Professor Maria do Carmo-Fonseca
Institute of Molecular Medicine
Faculty of Medicine
Universidade de Lisboa
1649-028 Lisboa Portugal
Phone:+351 21 794 0157
Fax:+351 21 795 1780
Email
Principal Investigators:
Walter Keller, University of Basel, CH
Torben Heick Jensen, University of Aarhus, DK
Jørgen Kjems, University of Aarhus, DK
Angela Krämer-Bilbe, Université de Genève, CH
Ulrike Kutay, Swiss Federal Institute of Technology (ETH), Zürich, CH
Marc Timmers, University of Utrecht, NL
ESF provides the scientific, administrative and technical secretariat for COST (European Cooperation in Science and Technology).